ORCA-Flash4.0 V3 Digital CMOS camera C13440-20CU

c13440-20cu product photo
Catalog (For lifescience) [939 KB/PDF]
Technical note [2 MB/PDF]

Building on our extensive experience with high performance scientific cameras and advanced imaging applications, Hamamatsu introduces the new ORCA-Flash4.0 V3. This one camera expertly handles applications ranging from the acquisition of beautiful scientific images to experiments that demand detection, quantification and speed. With on-board FPGA processing enabling intelligent data reduction, highly refined in-camera, pixel-level calibrations, increased USB3.0 frame rates, purposeful and innovative triggering capabilities, patented lightsheet read out modes and individual camera noise characterization the ORCA-Flash4.0 V3 is the precision instrument for imaging.

Special sites

The scientific camera section is the feature section for digital cameras fit to life science research and industry areas.

Special site for Scientific cameras


  • Calibrated for Quantitative Accuracy
  • Flexibility for Customized Data Control
  • Patented Tools for Advanced Imaging: Lightsheet Readout Mode
  • Focus on the Relevant Data
  • Powerful Triggering for Synchronizationa

Lightsheet Readout Mode [Patented]

“Lightsheet Readout Mode” is a unique and patented feature of Hamamatsu sCMOS cameras which can improve signal to noise ratios in Lightsheet microscopy.
For more information about the principle and features of Lightsheet Readout Mode, please see details from below.

What is Lightsheet Readout Mode?

Lightsheet Readout Mode

Movie gallery

3D animation of Amoeba scanned by DSLM

GFP-labeled zebrafish blood flow

High speed (400 fps) zebrafish cardiac beat

iPS cardiomyocyte membrane voltage dynamics (high resolution)

Localization microscopy acquisition

Spinning disk confocal pollen z scan images

Filopodial protrusion dynamics

High-speed intracellular Ca gradient driven by UTP stimulation

Microtubule growth dynamics TIRF

PKC translocation driven by TEA stimulation

High-speed Ca imaging of iPS cardiomyocyte

PC recommendations

With the introduction of the ORCA-Flash4.0, users are now able to stream 4 megapixel images to their computers 100 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Flash4.0.


Type number C13440-20CU
Quantum efficiency 82 % (peak QE: @560 nm)
Imaging device sCMOS
Effective no. of pixels 2048 (H)×2048 (V)
Cell size 6.5 μm×6.5 μm
Effective area 13.312 mm×13.312 mm
Full well capacity 30 000 electrons (typ.) *
Readout speed 100 frames/s (Full resolution, standard scan, Camera Link) *
40 frames/s (Full resolution, standard scan, USB 3.0, 16 bit) *
53 frames/s (Full resolution, standard scan, USB 3.0, 12 bit) *
80 frames/s (Full resolution, standard scan, USB 3.0, 8 bit) *
Readout noise Standard scan (at 100 frames/s, typ.):1.6 electrons rms (1.0 electrons median)
Slow scan (at 30 frames/s, typ.): 1.4 electrons rms (0.8 electrons median)
Cooling method Peltier cooling
Cooling temperature Forced air (Ambient at +20 ℃): -10 ℃
Water (+20 ℃): -10 ℃
Water (+15 ℃): -30 ℃
Dark current 0.06 electrons/pixel/s (Air Cooled to -10° C) (typ.)
0.06 electrons/pixel/s (Water Cooled to -10° C) (typ.)
0.006 electrons/pixel/s (Water Cooled to -30° C) (typ.)
Dynamic range 37 000:1 (typ.)
Interface Camera Link / USB 3.0
A/D converter 16 bit / 12 bit / 8 bit
Lens mount C-mount

* Typical value 2 Enabled with optional Camera Link board for PC


Readout Modes Normal Area, Lightsheet, W-VIEW Mode, Dual Lightsheet
Binning 2×2, 4×4
Master Pulse Generator (Pulse Modes) Internal Sync, Start Trigger, Burst
Master Pulse Generator (Pulse Interval in 1 μs increments) 10 μs to 10 s
Hot Pixel Correction Off, Low, Medium, High
Dark Signal Non-Uniformity (DSNU)* 0.3 electrons rms
Photo Response Non-Uniformity (PRNU) at half level of full light range (15,000 electrons)* 0.06 % rms
Photo Response Non-Uniformity (PRNU) at low light level (700 electrons)* 0.3 % rms
Linearity error, full light range (EMVA 1288 standard)* 0.5 %
Linearity error, low light range (< 500 electrons signal)* 0.2 % / Less than approx. 1 electron absolute error
On-camera Connectivity Both USB 3.0 and Camera Link**
V2 Compatibility Mode (for use with legacy software) Yes

*Typical value

**Enabled with optional Camera Link board for PC

Spectral response

C13440-20CU Spctral response


c13440-20cu dimensional outline

Instruction manual

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Disclaimer [66 KB/PDF]

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